Thrombin\PAR1 axis is associated with enhanced G-CSF stimulation in FVIII KO mice. (A) Thrombin blood plasma levels (nM) in FVIIIKO (gray) and C57BL (black) mice at different time points as measured by the TGA. Summary of the results is depicted on the right (n = 4). (B) The number of LSK cells in PB of G-CSF–treated C57BL/6 (black), PAR1KO (gray), and PAR2KO (white) mice. (C) CXCL12 protein BM plasma levels detected by ELISA with and without G-CSF treatment in C57BL (black), FVIIIKO (white), and PAR1KO (gray) mice. Data are represented in (ng/mL); n = 12 per group.