Gene-expression analysis of FOXP3 WT and null Tconv clones. Total RNA from T-cell clones was hybridized to a GeneChip Human Gene 1.0 ST array. (A) Hierarchical clustering revealed differential expression of 274 RefSeq annotated genes with a P < .05 (Benjamini correction). (B) Differentially expressed genes were subject to GSEA after cross-validation using randomization from background genes. (C) Differential expression of 6 genes was confirmed by quantitative RT-PCR. Mean values ± SEM determined in triplicate are shown.