American Society of Hematology

Figure 1

$Figure 1. Restoration of normal perforin expression in a fraction of hematopoietic cells restores perforin-dependent immune regulation in vivo. (A) Prf−/− mice were transplanted with WT (GFP marked) or prf−/− bone marrow. Twelve weeks later, they were infected with LCMV and serum IFN-γ levels were measured. N > 8 mice/point. (B) Mixed hematopoietic chimeric mice (prf−/− mice, reconstituted with mixtures of WT and prf−/− marrow) were challenged with LCMV, and day 8 serum IFN-γ levels were measured. (C) Six days after LCMV infection, splenic dendritic cells were sorted from groups of mixed chimeric mice (N = 3/group) and plated with LCMV-specific T cells. After overnight culture, IFN-γ production was assessed as a measure of antigen presentation. *P < .01 (comparing WT and prf curves in panel A; comparing the 2 upper curves with the lower 2 curves in panel C).$

Restoration of normal perforin expression in a fraction of hematopoietic cells restores perforin-dependent immune regulation in vivo. (A) Prf^−/− mice were transplanted with WT (GFP marked) or prf^−/− bone marrow. Twelve weeks later, they were infected with LCMV and serum IFN-γ levels were measured. N > 8 mice/point. (B) Mixed hematopoietic chimeric mice (prf^−/− mice, reconstituted with mixtures of WT and prf^−/− marrow) were challenged with LCMV, and day 8 serum IFN-γ levels were measured. (C) Six days after LCMV infection, splenic dendritic cells were sorted from groups of mixed chimeric mice (N = 3/group) and plated with LCMV-specific T cells. After overnight culture, IFN-γ production was assessed as a measure of antigen presentation. *P < .01 (comparing WT and prf curves in panel A; comparing the 2 upper curves with the lower 2 curves in panel C).

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