Influx of 2 subpopulations of VE-cadherin BMDCs. (A) Representative confocal pictures show EYFP⁺ cells in the LLC tumors (blue: TO-PRO, red: PECAM, green: EYFP). (B) Shown are adult bone marrow cells expressing VE-cadherin from 2 distinct populations: an endotheliallike population expressing PECAM and VE-cadherin and a monocytic population expressing Gr1 and CD11b. (C) Cytospins of FACS sorted BMD-EYFP⁺ cells stained for Gr1, PECAM, and VE-cadherin are shown. (D-E) The graphs show the percentage of (D) EYFP⁺/PECAM⁺ or (E) EYFP⁺/PECAM⁺/CD45⁻ cells in subcutaneous growing LLC cells in BL/6 mice transplanted with CIVE bone marrow 8 days after the start of treatment. (F-G) The graphs show the percentage of (F) EYFP⁺/Gr1⁺/CD11b⁺ cells or (G) Gr1⁺/CD11b⁺ cells in subcutaneous growing LLC cells in BL/6 mice transplanted with CIVE bone morrow 1 day after the start of treatment. *P < .05 compared with the vehicle control. *, single green EYFP cells; arrowheads, double-positive cells (EYFP⁺/PECAM⁺). All images were obtained at 20× except for the zoom images; scale bar sizes are indicated.