YOD1-C160S mice mount enhanced antigen-specific CD8⁺ T-cell response on immunization. (A) Schematic of in vivo experiments is shown. Control and YOD1-C160S mice, which either received OT-I cells or no cells, were immunized subcutaneously with 200 μg of Ova. Five × 10⁵ OT-I cells were transferred into control and YOD1-C160S mice, which were then immunized subcutaneously with 200 μg of Ova in IFA. Frequencies and numbers of K^b-SIINFEKL-Tet⁺ CD8⁺ T cells in draining iliac LNs after 7 days were measured by flow cytometry. (B) Representative FACS plots show the frequencies of K^b-SIINFEKL-Tet⁺ cells. (C) Absolute numbers of K^b-SIINFEKL-Tet⁺ cells in draining iliac LNs of 2 groups of mice are shown. (D-E) Control and YOD1-C160S mice were immunized subcutaneously with 200 μg of Ova in incomplete Freund adjuvant (IFA) and the frequencies and numbers of endogenous K^b-SIINFEKL-Tet⁺ cells were analyzed in iliac LN 7 days after transfer. (D) Representative FACS plots show frequencies of endogenous K^b-SIINFEKL-Tet⁺ cells. (E) Total numbers of K^b-SIINFEKL-Tet⁺ cells in the iliac LNs of control and YOD1-C160S mice are shown. In each experiment, 4 mice were included and experiments were repeated at least 3 times. Mean values ± SEM are shown.