Peptide length has a major impact on TCR degeneracy. (A) 1E6 CD8⁺ T-cell recognition of a set of 30 11mer peptides sampled by CPL-based importance. (B) 1E6 CD8⁺ T-cell recognition of a set of 30 12mer peptides sampled by CPL-based importance. Assays were conducted as described in Figure 3. (C) CPL-based importance sampling was used to construct a degeneracy curve that describes the number of 11mer and 12mer peptides that can be recognized by the 1E6 TCR. The ordinate is the estimated number of peptides with a functional sensitivity of at least the value indicated on the abscissa. Error bars represent SDs.