Figure 7
Figure 7. Neutrophils mobilized by Am80 in neutropenic mice display greater bactericidal activity than those by G-CSF. Twenty C57BL6/J mice were randomly divided into 4 groups for the experiments. (A) G-CSF induced a remarkably accelerated neutrophil recovery compared with mice treated with Am80 or vehicle at day 5. (B) Morphologic analysis of PB neutrophils at day 5 (*G-CSF versus Am80, P < 1.2 × 10−6; G-CSF versus control, P < 4.0 × 10−5; G-CSF versus vehicle, P < 8.2 × 10−8; Am80 versus vehicle, P < 1.3 × 10−6; Am80 versus control, P < .016. (C) Representative purity of PB neutrophils, as shown by freshly isolated GINs from mice. (D) Phagocytotic and bactericidal activities of neutrophils, isolated from PB of different mice, were reflected by the number of extracellular bacteria, phagocytized bacteria, and killed bacteria. *Extracellular: AINs versus GINs, P < .043; AINs versus C-MPBNs, P < 1.1 × 10−4; MPBNs versus C-MPBNs, P < 3.7 × 10−4; GINs versus C-MPBNs, P < .049. *Phagocytosis: AINs versus GINs, P < .026; AINs versus C-MPBNs, P < .02; MPBNs versus GINs, P < .042; MPBNs versus C-MPBNs, P < .003; GINs versus C-MPBNs, P < .009. *Killing: AINs versus GINs, P < .026; AINs versus C-MPBNs, P < .005; MPBNs versus GINs, P < .015; MPBNs versus C-MPBNs, P < .003; GINs versus C-MPBNs, P < .009. (E) Accelerated recoveries of WBC and neutrophil were ceased at day 7 after 96 hours of stimuli with G-CSF or Am80 or vehicle. (F) Morphologic analysis of PB neutrophils at day 9 (*G-CSF versus control, P < .005; Am80 versus control, P < 3.9 × 10−4; Am80 versus vehicle, P < .03; Vehicle versus control, P < .03). (G) AINs possessed significantly higher phagocytotic and bactericidal activities than do GINs 48 hours after cessation of accelerated neutrophil recovery (E). *Extracellular: AINs versus GINs, P < .02; AINs versus C-MPBNs, P < .007; MPBNs versus GINs, P < .04; MPBNs versus C-MPBNs, P < .02. *Phagocytosis: AINs versus C-MPBNs, P < .034; MPBNs versus C-MPBNs, P < .043. *Killing: AINs versus C-MPBNs, P < .034; MPBNs versus C-MPBNs, P < .043.

Neutrophils mobilized by Am80 in neutropenic mice display greater bactericidal activity than those by G-CSF. Twenty C57BL6/J mice were randomly divided into 4 groups for the experiments. (A) G-CSF induced a remarkably accelerated neutrophil recovery compared with mice treated with Am80 or vehicle at day 5. (B) Morphologic analysis of PB neutrophils at day 5 (*G-CSF versus Am80, P < 1.2 × 10−6; G-CSF versus control, P < 4.0 × 10−5; G-CSF versus vehicle, P < 8.2 × 10−8; Am80 versus vehicle, P < 1.3 × 10−6; Am80 versus control, P < .016. (C) Representative purity of PB neutrophils, as shown by freshly isolated GINs from mice. (D) Phagocytotic and bactericidal activities of neutrophils, isolated from PB of different mice, were reflected by the number of extracellular bacteria, phagocytized bacteria, and killed bacteria. *Extracellular: AINs versus GINs, P < .043; AINs versus C-MPBNs, P < 1.1 × 10−4; MPBNs versus C-MPBNs, P < 3.7 × 10−4; GINs versus C-MPBNs, P < .049. *Phagocytosis: AINs versus GINs, P < .026; AINs versus C-MPBNs, P < .02; MPBNs versus GINs, P < .042; MPBNs versus C-MPBNs, P < .003; GINs versus C-MPBNs, P < .009. *Killing: AINs versus GINs, P < .026; AINs versus C-MPBNs, P < .005; MPBNs versus GINs, P < .015; MPBNs versus C-MPBNs, P < .003; GINs versus C-MPBNs, P < .009. (E) Accelerated recoveries of WBC and neutrophil were ceased at day 7 after 96 hours of stimuli with G-CSF or Am80 or vehicle. (F) Morphologic analysis of PB neutrophils at day 9 (*G-CSF versus control, P < .005; Am80 versus control, P < 3.9 × 10−4; Am80 versus vehicle, P < .03; Vehicle versus control, P < .03). (G) AINs possessed significantly higher phagocytotic and bactericidal activities than do GINs 48 hours after cessation of accelerated neutrophil recovery (E). *Extracellular: AINs versus GINs, P < .02; AINs versus C-MPBNs, P < .007; MPBNs versus GINs, P < .04; MPBNs versus C-MPBNs, P < .02. *Phagocytosis: AINs versus C-MPBNs, P < .034; MPBNs versus C-MPBNs, P < .043. *Killing: AINs versus C-MPBNs, P < .034; MPBNs versus C-MPBNs, P < .043.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal