Both unsorted and sorted AINs display a higher capacity for clearance of bacteria than do unsorted and sorted GINs. (A) Morphology of GINs and AINs before and after sorting with anti-CD15 antibody. Arrows indicate examples of monocytes. (B) Morphology of freshly isolated PBNs. (C-D) Quantification of monocytes (C) and band/segmented neutrophils (D) in panels A and B. (E-F) Comparison of the effect of unsorted and sorted GINs and AINs on the clearance of intracellular bacteria. Clearance efficiency was determined from the numbers of viable bacteria recovered from the intracellular compartment after infection. E coli DH5α at an MOI of 100 were used to infect sorted GINs, sorted AINs, and PBNs (F). (G) In situ labeling of bacteria with anti-OmpA antibodies in unsorted GINs and AINs.