AINs produce and secrete granules more effectively than do GINs. (A-C) Ultrastructural images of GINs (A), AINs (B), or freshly isolated PBNs (C) with electron microscope. The vesicles in GINs appeared to contain less dense and amorphous material, whereas those in AINs showed dense material or both dense and amorphous material. AINs appeared to have more primary and secondary-like granules than do GINs. Arrows indicate examples of vesicles and granules. Ve indicates vesicle; Pg, primary granule; and Sg, secondary granule. (D) NE activity challenged by fMLP stimuli. CB indicates cytochalasin B. (E) AINs had a greater production and secretion of the cleaved 38-kDa product of MPO upon E coli stimuli. Images at the top of the horizontal line were derived from 8% gel, whereas images under the horizontal line from 12% gel. The band intensities of Actin detected in 12% gel are similar to those in 8% gel (data not shown). (F) Effective secretion of lactoferrin by AINs versus GINs upon E coli stimuli. To make the sample orders in the medium section match those in the lysate section, the positions between nonstimulated and stimulated samples in the medium section have been switched, as indicated by a vertical line. (G) Greater production and degranulation of LL37 by AINs versus GINs. (H) Increased abundance of intracellular MMP-9 upon E coli stimuli but insufficient degranulation in both GINs and AINs. To make the sample orders in the medium section match those in the lysate section, the positions between nonstimulated and stimulated samples in the medium section have been switched, as indicated by a vertical line. In addition, the samples loaded in lysate and medium sections in panels E through H were originally separated by a molecular weight (MW) marker. This MW marker was deleted so that a blank space is shown between lysate and medium samples.