IκB-ζ downregulation deregulates NF-κB activity in ABC DLBCL. (A) Gene expression profiling after IκB-ζ knockdown in HBL-1 cells. Changes of gene expression were profiled at the indicated time points after induction of IκB-ζ shRNA 1. Gene expression changes are depicted according to the color scale shown. Known NF-κB target genes and genes involved in processes such as inflammation are highlighted. (B) Gene set enrichment analysis of the MLN120b-defined NF-κB gene expression signature. The NF-κB signature is significantly downregulated after IκB-ζ knockdown. (C) IκB-ζ knockdown downregulates NF-κB target genes in HBL-1, OCI-Ly3, and OCI-Ly10, as measured by quantitative PCR. Error bars indicate the standard deviation. (D) IκB-ζ knockdown downregulates IL-10 in IL-10-expressing HBL-1 and IL-6 in IL-6-expressing OCI-Ly3 cells measured by ELISA. Error bars indicate the standard deviation. (E) IκB-ζ knockdown downregulates NF-κB targets in HBL-1 and OCI-Ly10, as measured by western blotting. N.S., not significant; *P < .05; **P < .01; ***P < .001.