DEPTOR regulates the expression of proinflammatory chemokines and adhesion molecules in ECs. mRNA array analysis (supplemental Table 2) identified 27 genes that were induced in expression in DEPTOR siRNA-transfected EC vs controls. Of these, 8 represented established EC activation response genes, members of the chemokine and adhesion molecule families. (A) qPCR was performed on control or 2 DEPTOR siRNA-transfected ECs after 48 hours to validate the function of DEPTOR on the expression of the 8 genes, CXCL9 (MIG), CXCL10 (IP-10), CXCL11 (I-TAC), CX3CL1 (Fractalkine), CCL5 (RANTES), and CCL20 (MIP-3A), and the adhesion molecules VCAM-1 and intercellular adhesion molecule-1. Graphs represent the mean fold change in mRNA expression (±SEM) in 3 independent experiments. (B) Average fold induction (high to low) in mRNA expression of each gene, as indicated, in DEPTOR siRNA-transfected EC vs controls. (C) HUVECs were transfected with control or 2 DEPTOR siRNAs, and the concentration of IP-10 protein was evaluated by ELISA in culture supernatants after 96 hours. Control siRNA-transfected HUVECs treated with interferon γ (1000 U/mL) for 24 hours were used as positive control. The bar graphs illustrate the mean IP-10 concentration (±SEM) from 3 experiments (*P < .05, **P < .01, ***P < .001).