Clinical scale HA-1 TCR2-RQR-CD8–transduced T cells retain functional recognition of HA1⁺target cells. Functional evaluation of HA-1 TCR2-RQR-CD8–transduced CD8⁺ and CD4⁺ T cells in the final T-cell product. (A) Lysis of target T2 cells pulsed with a range of VLH (blue) and VLR (green) peptide concentrations by CD8⁺ (solid lines) and CD4⁺ T cells (dashed lines) in CRAs at an E:T ratio 20:1. (B) Lysis of HA-1⁺ A2⁺ LCL (blue), HA-1^– A2⁺ LCL (green), and AML HA1⁺ A2⁺ cell lines (THP-1; gray) by CD8⁺ and CD4⁺ T cells in CRAs. (C) IL-2, IFN-γ, and tumor necrosis factor–α production by T cells in response to stimulation by T2 cells pulsed with 10 ng/mL of HA-1 peptide. (D) Pie charts displaying the number of cytokines secreted by T cells. (E) Concentration of cytokines and granzyme B in media 24 hours after stimulation of T cells by T2 cells pulsed with VLH (blue) and VLR (green) peptides measured by multiplex immunoassay.