Evaluation of clinical-scale iC9-HA-1TCR2-RQR-CD8–transduced T cells. Evaluation of iC9-HA-1TCR2-RQR-CD8–transduced (transduced) CD8⁺ (left) and CD4⁺ (right) T cells. (A) Growth of transduced T cells. (B) HA-1 TCR multimer binding and CD34 expression on transduced T cells by flow cytometry with HA-1/HLA-A2 multimer staining. (C) Expression of costimulatory and homing molecules on T cells at the time of the apheresis (gray), after CD45RA depletion (light green), and after transduction and expansion (blue) (n = 5). (D-E) Expression of “exhaustion” markers on HA-1 TCR CD8⁺ and CD4⁺ in the final cell product (n = 3) (D) and a representative example (E).