Evaluation of CD8⁺T cells transduced with constructs, including HA-1 TCR2, and safety switch and/or CD8 coreceptor and/or a selection marker. (A) Drawing illustrating the composition of the 5 different LV transgene constructs evaluated: (i) iCasp9(iC9)-HA1 TCR2; (ii) HA1 TCR2-CD8 coreceptor (CD8); (iii) iC9-HA1 TCR2-CD8; (iv) iC9-HA1 TCR2-RQR-CD8; and (v) iC9-CD34-HA1 TCR2-CD8. (B) Intracellular cytokine staining showing production of IL-2 and IFN-γ by CD8⁺ and CD4⁺ T cells transduced with the LV transgene constructs in response to HLA-A2/HA-1⁺ primary AML. (C) CRAs showing specific lysis of HLA-A2⁺/HA-1⁺ AML (AML 1-4) or HLA-A2⁺/HA-1^– AML (AML 5) by CD8⁺ T cells transduced with the LV transgene constructs.