Flow chamber and fluid velocity simulation. (A) Schematic of the flow chamber platform used for analysis. Cells were seeded on a DropArray plate, and flow chamber was overlaid on top of the cells and DropArray plate. (B) Quantification of the percentage of cells remaining in the well after 30 minutes of constant exposure to fluid velocities of 2.5, 5.0, 10, and 20 mm/s and the standard error. As the fluid velocity increases, the number of cells retained decreases. (C) Time series micrographs of K562 cells seeded within a 2-mm droplet during exposure of constant fluid sheer force (2.5 and 20 mm/s) over 30 minutes. Scale bar = 0.5 mm. (D) Visualization of simulated fluid movement within the chamber. Before the application of horizontal washing action of 44 RPM, the air (blue) and liquid media (red) span the length of the chamber. During washing, the fluid and air components oscillate between the ends of the chamber. (E) Quantification of simulated fluid velocity with a washing speed of 44 rpm at 10 μm above the chamber floor. The average fluid velocity (mm/s) at each location along the chamber floor is plotted as well as the standard error over the duration of washing (10 seconds). In addition, the maximum velocity at each location is plotted, representing the fastest fluid movement a cell on the chamber floor could potentially be exposed.