LSK cells isolated from the BM of WT but not TLR2 knockout mice undergo granulopoiesis in response to in vitro Pam₃CSK₄ stimulation. A total of 5000 FACS LSK cells were plated per well and were stimulated with 1 µg/mL of the TLR2 agonist Pam₃CSK₄ or vehicle control. Cells were enumerated at day 7. PMN number (A) and percentage (B) for each well was determined by anti-Ly6G staining and flow cytometry. Data represent 3 mice per group and are shown as mean ± SEM (*P < .05).