Cellular uptake of microvesicles from GM-CSF–stimulated monocytes. Donor monocytes cells were stained with D384 and SytoRNA Select then treated with GM-CSF. Microvesicles from GM-CSF–stimulated monocytes were collected and added to different cells lines as indicated. Representative confocal images using the Zeiss LSM 510 multiphoton confocal microscope are shown. Microvesicles obtained from 3 individual monocyte donors revealed the uptake of the microvesicles by (A) A549 lung epithelial cells, (B) CCL-204 lung fibroblasts, and (C) human umbilical vein endothelial cells (HUVECs). (D) Quantification of microvesicle transfer from 3 independent experiments is shown (average ± SEM). Cellular morphology was visualized without fluorescence using differential interference contrast (DIC).