Gene expression profiling after microvesicle treatment. Microarray was performed using Human Genome U133 Chips from RNA isolated from THP-1 cells (n = 3) and peripheral blood monocytes from 3 individual monocyte donors to 3 different monocyte recipients. Each treatment was performed in 3 independent experiments. (A) Principle component analysis (PCA) mapping between THP-1 cells treated with vehicle (DMSO), PMA, or microvesicles from PMA-stimulated cells. (B) Freshly isolated monocytes treated with GM-CSF or microvesicles from GM-CSF–stimulated monocytes were compared with freshly isolated monocytes by PCA. THP-1 cells treated with PMA or microvesicles from PMA-stimulated THP-1 cells expressed similar genes and were different from vehicle-treated cells. Gene expression was similar in monocytes incubated with GM-CSF or microvesicles from GM-CSF–stimulated cells but different compared with freshly isolated monocytes. (C) Venn diagrams generated using pairwise comparisons for significantly expressed genes in cells treated as indicated showed 126 significantly coexpressed genes between THP-1 cells treated with PMA or microvesicles from PMA-stimulated THP-1 cells. (D) 407 significantly coexpressed genes were found between the monocytes incubated with either GM-CSF or microvesicles from GM-CSF–stimulated monocytes.