Local ablation of lymphatic vessels after intradermal injection of DT into the ear skin. (A) Diagram of experimental scheme for local lymphatic ablation by intradermal injection of DT (10 ng/mL) into the left ear skin of LCiDT mice. Samples were harvested at 7 days after triple injections of DT. (B) Images showing LYVE-1+ lymphatic vessels and CD31+ blood vessels in the left and right ear skins of LCiDT and control mice. Scale bars, 100 μm. (C) Quantifications for LYVE-1+ area in the ear skins. Each group, n = 4. *P < .05 vs control. (D) Images showing LYVE-1+ lymphatic vessels in draining cervical lymph node (cLN) and distal inguinal lymph node (iLN) of LCiDT and control mice. Scale bars, 100 μm. (E) Quantifications for LYVE-1+ area in the LNs. Each group, n = 4. *P < .05 vs cLN of control. (F) Lymphatic drainage of Evans blue dye in the ear skins. The dotted line shows the Evans blue injection site. The arrowhead indicates no lymph drainage. Similar findings were observed in 3 to 4 different mice. (G) Images showing LYVE-1+ lymphatic vessels in jejunums. Scale bars, 100 μm. (H) Quantifications for LYVE-1+ area in the jejunums. Each group, n = 4. NS, not statistically significant. DAPI, 4,6 diamidino-2-phenylindole.