Abnormal lymphatic functions of LYVE-1-Cre/iDTR mice after DT administration. Comparisons between control and LCiDT mice for major findings at 24 hours after DT administration. Each group, n = 4–5. *P < .05. (A) Popliteal lymph nodes after injection of fluorescein isothiocyanate–conjugated lectin into the footpad. Scale bars, 200 μm. (B) Quantification of the lectin-drained area density inside the popliteal lymph nodes. (C) Distributions of CD11c+ dendritic cells inside of the inguinal lymph nodes. Indicated areas (square with white dotted line) are magnified in the right panel. The white asterisk indicates abnormally distributed CD11c+ dendritic cells in the B-cell zone. Scale bars, 100 μm. (D) Distributions of CD169+ sinusoidal macrophages in the subscapular and medullary sinuses of the inguinal lymph nodes. The yellow asterisks indicate abnormally distributed CD169+ sinusoidal macrophages. Scale bars, 100 μm. (E) Lymphatic drainage of Evans blue dye in the ear skins. Inside dotted line, Evans blue injection site. The arrowhead indicates leakage areas of skin lymphatic vessels. (F) Quantification of the number of leakage points of Evans blue dye. DAPI, 4,6 diamidino-2-phenylindole.