Figure 2
![Figure 2. Ligase activity-deficient COP1 mutants fail to induce degradation of C/EBPα and block granulocytic differentiation. (A) Schematic representation of ligase activity-deficient COP1 mutants. (B) Lysates from GFP-positive 32D cells infected with viruses containing GFP, GFP-COP1 (WT and mutants [CS and ΔRING]) were analyzed by western blotting using antibodies against COP1, C/EBPα, and γ-tubulin. (C) 32D transfectants (GFP, COP1 WT and mutants) prepared in B were maintained in IL3, transferred to G-CSF for 4 days, and analyzed for expression of Mac-1 by flow cytometer. Results shown in B and C are representative of 2 independent experiments. CS, an abbreviation of C138S/C141S.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/122/10/10.1182_blood-2012-12-476101/4/1750f2.jpeg?Expires=1723740528&Signature=QZ7pJOYaRACtK1CpgEVLCE1-6twl03Ld-F5wUj-37M5BUDyad~f76YFIrvLrdHJ8yj6prdtB2oVmc1vhXws6BtFrcpRY-upprx2sqsNq0AFHKA6SVCk2lFaRDUSk1tb-Pi8l7gG-s2jA8bq-PBDnXYrywX~Ne3ReAq9jbJWh-qVvHXeifiE-cnszr6KnrrwFyVHE0Jv1cWetn3LpH8h4gdL6rEiDsoStD07IrXEx4-jo1iI-7DPKlCfkAbQmFitdnnDatopD6SrdwHRO9tUSlsvEkjVabeY7YsgyFMJaU5ZWQ0VjK4CHOAcvdmTj0IwfOTDnob-gHy10Gcm~r4ByBg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Ligase activity-deficient COP1 mutants fail to induce degradation of C/EBPα and block granulocytic differentiation. (A) Schematic representation of ligase activity-deficient COP1 mutants. (B) Lysates from GFP-positive 32D cells infected with viruses containing GFP, GFP-COP1 (WT and mutants [CS and ΔRING]) were analyzed by western blotting using antibodies against COP1, C/EBPα, and γ-tubulin. (C) 32D transfectants (GFP, COP1 WT and mutants) prepared in B were maintained in IL3, transferred to G-CSF for 4 days, and analyzed for expression of Mac-1 by flow cytometer. Results shown in B and C are representative of 2 independent experiments. CS, an abbreviation of C138S/C141S.
