CD4⁺ T-cell priming defect in miR-142^−/− mice. (A) Schematic of the experimental protocol. (B) Flow cytometric analysis of T-cell grafts retrieved from immunized recipient mice indicating proliferated OT-I CD8⁺ T cells (left) and OT-II CD4⁺ T cells (right) cells in miR-142^+/− (red) and miR-142^−/− mice (blue). (C) Quantification of CFSE mean fluorescence intensity (MFI) of proliferated OT-I and OT-II cells. OT-II CFSE MFI WT 14 473 ± 4961 versus CFSE MFI knockout (ko) 31 812 ± 2316, P = .005. Each dot represents an animal. *P < .05 was considered significant using a Student 2-tailed t test. (D) Flow cytometric analysis of splenic CD4⁺ DCs isolated from miR-142^−/− mice and littermate controls for the cell-surface molecule ESAM. Cells were gated on CD11c⁺ MHCII^hi and CD4⁺. Each dot represents an animal.