Dll4 blockade rescues aberrant HSC/lymphoid development in Lrf knockout mice in vivo. (A) Treatment schedule: 9 dosages of anti-Dll4 antibody were administered intraperitoneally over 4 weeks. Mice were treated with pIpC (250 μg/mouse) on days 1, 4, and 7. (B) Hematopoietic development in the BM was analyzed on day 28. Representative FACS profiles for each group are shown. (C) Dot graph shows proportions of DP T (top) and pre-B cell compartments (bottom) in BM. Horizontal black bars: average value; error bars: standard deviation. (D) Dot graph shows numbers of LT-HSCs per 2 legs on day 28. Horizontal black bars: average value; error bars: standard deviation.