Ultrastructural features of CIP4-null MKs reveal reduced platelet territories. (A) TEM (JEM 1011, JEOL, Tokyo, Japan) analysis of bone marrow megakaryocytes from WT (left) vs CIP4 KO (right) mice revealed greatly reduced to completely missing platelet territories in CIP4 KO megakaryocytes. The DMS, which are membranes separating platelet territories, were either poorly defined or missing altogether in the CIP4 KO megakaryocytes. A minimum of 3 mice was analyzed for each group. Shown are representative images for each group. Magnification as mentioned on the figure; bar represents 2 um. Areas within the boxes are shown in the lower panel at a higher magnification. (B) Ultrastructural features of CHRF-288-11 cells. Bottom images: signs of membrane disruption of the plasma membrane and vacuole membrane are evident in cells with CIP4 knockdown. Bottoms left 2 images: the plasma membrane is clearly disrupted in the CIP4 KD CHRF-288-11 cell, whereas a vacuole with protruding vesicles is seen in another cell (bottom right 2 images). Areas within the boxes are shown on the right adjacent side at a higher magnification.