Tregs derived from different tissues of healthy human donors migrate to different chemokines. Flow cytometry of homing marker expression and migratory capacity of CD4^posCD25^posCD127^negCD69^pos Tregs and CD4^posCD25^posCD127^negCD69^neg in human SPL and PB samples. (A) Percentages of Tregs positive for indicated markers are shown (N = 3 for each tissue). Data were analyzed using a random effect logistic regression model. Significant differences are indicated: *P < .05; **P < .01; ***P < .001. (B) Migration assays were performed using a transwell system, with the indicated recombinant human chemokines present in the lower compartment. The migratory capacity of the Treg subsets is shown (mean plus SD, N = 3 for each tissue). Data were compared using 1-way ANOVA. Significant differences are indicated: *P < .05; **P < .01; ***P < .001.