ETS1 and FLI1 down-regulation in other DLBCL cell lines. (A) ETS1 and FLI1 down-regulation in SU-DHL4, Val, U-2932, SU-DHL2, and OCI-Ly10 DLBCL cell lines. Real-time PCR and western blot analysis of ETS1 and FLI1 levels after shRNA (day 5). As a control, β2-microglobulin mRNA and GAPDH protein expression was documented. (B) Apoptosis in 5 different DLBCL cell lines after ETS1 and FLI1 down-regulation. Percentage of apoptotic cells was determined by annexinV staining at day 5. Histogram graphs represent the average of at least 2 independent experiments.*P value < .05. (C) For the growth curve, SU-DHL2 cells infected with control (shGFP) lentivirus or a lentivirus expressing shRNA for ETS1 and FLI1 (shETS1 and shFLI1, respectively) were seeded on 24-well plates at a density of 105 cells/well. Cultures were harvested every day and the number of cells was determined. The numbers refer to mean values of triplicate determinations.
