Competitive transplantation of Notch-activated HSPCs leads to progressive exhaustion of HSC activity and numbers. A similar experiment was performed as in Figure 4A but with the addition of 200 000 whole CD45.1⁺ BM cells to prevent mortality from hematopoietic failure. (A) BM DN T-cell production (Thy-1⁺CD25⁺) in MigR1 control and Notch mice was measured in the BM at 2 and 16 weeks after transplantation. (B) Scatter plot analysis of DN T-cell production by MigR1 controls and Notch mice at 2 and 16 weeks after transplantation. (C) The LT-HSC compartment (Lineage⁻Sca-1⁺c-Kit⁺CD34⁻Flt3⁻) was measured for donor reconstitution by GFP⁺ MigR1 control and Notch-activated LT-HSCs at 2 and 16 weeks after transplantation. (D) Scatter plot analysis of donor-derived (GFP⁺) LT-HSCs (Lineage⁻Sca-1⁺c-Kit⁺CD34⁻Flt3⁻) at 2 and 16 weeks after transplantation. Experiment was performed 3 times. MigR1, 6 mice at 2 weeks; 5 mice at 16 weeks. Notch, 3 mice at 2 weeks; 6 mice at 16 weeks.