American Society of Hematology

Figure 1

$Figure 1. Simultaneous genotyping for JAK2V617F and BCR-ABL1 of individual CFU-GM, BFU-E, and mixed CFUs assayed in semisolid medium and in colonies generated from single CD34+ cells in liquid cultures. CD34+ cells were assayed in semisolid medium (A) or in single-cell liquid cultures (B), supplemented with SCF, thrombopoietin, IL-3, IL-6, and G-CSF, each at 100 ng/mL, and 4 units/mL of erythropoietin (Amgen). Individual HCs were plucked from semisolid media or from liquid cultures of single CD34+ cells. The colonies were divided into 2 parts. Half were analyzed for JAK2V617F using a nested allele-specific PCR and the other half were analyzed for the presence of BCR-ABL1 using interphase FISH. Each HC contained either heterozygous or homozygous JAK2V617F, whereas the BCR-ABL1 was demonstrated only in a fraction of these HCs. JAK2 wild-type HCs and BCR-ABL1+ HCs without JAK2V617F were not observed in either patient.$

Simultaneous genotyping for JAK2V617F and BCR-ABL1 of individual CFU-GM, BFU-E, and mixed CFUs assayed in semisolid medium and in colonies generated from single CD34⁺ cells in liquid cultures. CD34⁺ cells were assayed in semisolid medium (A) or in single-cell liquid cultures (B), supplemented with SCF, thrombopoietin, IL-3, IL-6, and G-CSF, each at 100 ng/mL, and 4 units/mL of erythropoietin (Amgen). Individual HCs were plucked from semisolid media or from liquid cultures of single CD34⁺ cells. The colonies were divided into 2 parts. Half were analyzed for JAK2V617F using a nested allele-specific PCR and the other half were analyzed for the presence of BCR-ABL1 using interphase FISH. Each HC contained either heterozygous or homozygous JAK2V617F, whereas the BCR-ABL1 was demonstrated only in a fraction of these HCs. JAK2 wild-type HCs and BCR-ABL1⁺ HCs without JAK2V617F were not observed in either patient.

This Feature Is Available To Subscribers Only