Figure 5.
![Figure 5. The degree of antibody opsonization correlates with the degree of antibody-mediated internalization of PLTs by macrophages. PLTs from healthy individuals were incubated with 2 antibodies (anti-HPA1a [A,C] and anti-pan HLA class I [B,D]) in increasing concentrations (0.1 μg/mL, 1 μg/mL, and 10 μg/mL). As negative controls, PLTs were left unopsonized. Representative plots of 3 individual experiments are depicted (A-B). PLTs were incubated with macrophages, and PLT internalization was quantified. (C-D). A Pearson correlation test was used to determine the association between PLT antibody opsonisation and PLT internalization by macrophages.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/131/1/10.1182_blood-2017-07-799270/4/blood799270f5.jpeg?Expires=1723739164&Signature=SFlsVOl6JFK3oRSc1U9mW0Rj2EffGG1-39tSOAFZPaT2BgUMj8~A0JLwYwoGzM0gekIdD0vT~sRFQk20jlelkgH1Byav8FmXNxrbosQmoMVuaLh~8gfw2o~IUW5~QC3BQ~dCEPysv5N~ZIbTXgEJi~A1meKxW6-sEdSnheCYU5mkG0cpl59cYaqgdxE0ibjH3fc2rpWNrUCSFkvimloPJ3mQa7pkMIKyDv477CKpqp3nBgXpQbPuUzVFDvchqMr3RJdihj7ZDtrbrLFNvCA~wI05hk~1DyZD8In53Xf5cOWdTazgWPyoGjim9eJV-Nn8kTDigprPOm-8nQxVejK0VA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The degree of antibody opsonization correlates with the degree of antibody-mediated internalization of PLTs by macrophages. PLTs from healthy individuals were incubated with 2 antibodies (anti-HPA1a [A,C] and anti-pan HLA class I [B,D]) in increasing concentrations (0.1 μg/mL, 1 μg/mL, and 10 μg/mL). As negative controls, PLTs were left unopsonized. Representative plots of 3 individual experiments are depicted (A-B). PLTs were incubated with macrophages, and PLT internalization was quantified. (C-D). A Pearson correlation test was used to determine the association between PLT antibody opsonisation and PLT internalization by macrophages.
