American Society of Hematology

Figure 2

OVA-presenting dendritic cells develop after transfer of 11c.OVA BM. (A-E) B6.SJL (CD45.1 congenic) mice were irradiated (300 cGy) and BM (107) from C57Bl/6 (non-Tg), 11c.OVA, MII.OVA, or actin.OVA (act.OVA) mice injected intravenously. At the designated timepoints PBL were analyzed by flow cytometry (A-D). Four weeks after BM transfer CFSE-labeled CD45.1+ OT-I cells were transferred intravenously to non-Tg and 11c.OVA BM recipients. Three days later LNs were recovered and CFSE dilution in OT-I (CD45.1+CD8+) T cells were assessed by flow cytometry. Data are mean ± SD (n = 4-10) pooled from 2 or more experiments (A-D) or representative of 2 mice analyzed in 2 separate experiments (E).

OVA-presenting dendritic cells develop after transfer of 11c.OVA BM. (A-E) B6.SJL (CD45.1 congenic) mice were irradiated (300 cGy) and BM (10⁷) from C57Bl/6 (non-Tg), 11c.OVA, MII.OVA, or actin.OVA (act.OVA) mice injected intravenously. At the designated timepoints PBL were analyzed by flow cytometry (A-D). Four weeks after BM transfer CFSE-labeled CD45.1⁺ OT-I cells were transferred intravenously to non-Tg and 11c.OVA BM recipients. Three days later LNs were recovered and CFSE dilution in OT-I (CD45.1⁺CD8⁺) T cells were assessed by flow cytometry. Data are mean ± SD (n = 4-10) pooled from 2 or more experiments (A-D) or representative of 2 mice analyzed in 2 separate experiments (E).

This Feature Is Available To Subscribers Only