FeCl₃-induced thrombosis increased expression of TF in WT but not in Gas6^−/− mice. (A) Representative immunofluorescence staining for TF in the IVC of the 4 groups of mice generated by the platelet depletion/reconstitution experiments (original magnification ×40). Endothelial cells were stained using CD31-AlexaFluor 488-nm antibodies (green). Smooth muscle cells were stained with α-smooth muscle actin (αSMA) antibody coupled with Cy3 dye (red). TF was stained with a specific primary antibody followed by AlexaFluor 405-nm (blue). (B) Quantifications of TF expression were done in the vascular wall, delimited by the endothelial cells and the smooth muscle cells. TF expression was decreased in the vascular wall of the Gas6^−/− → Gas6^−/− group compared with the WT → WT. Interestingly, expression of TF was intermediate in the 2 chimeric groups but not statistically different from the Gas6^−/− → Gas6^−/− or WT → WT groups (n = 4 or 5 per group). *P < .05.