Contribution of vascular Gas6 to thrombus generation. (A) Success of marrow uptake after irradiation/transplantation was determined by Gas6 PCR from DNA isolated from peripheral blood cells (hematopoietic) and ear (nonhematopoietic). Lanes 1 and 4 indicate nonhematopoietic DNA from ears of WT mouse before transplantation; lanes 2 and 5, DNA from ears of WT mouse transplanted with bone marrow from Gas6^−/− mice; lanes 3 and 6, hematopoietic DNA from blood of WT mice transplanted with bone marrow from Gas6^−/− mice; lane 7, DNA from blood of a WT mouse transplanted with bone marrow from a WT mouse; lane 8, DNA from the ear of a WT mouse; and lane 9, DNA from the ear of a Gas6^−/− mouse. (B) Thrombosis was induced in the 4 groups of mice generated by the bone marrow transplantation experiments. Thrombus weight from chimeric mice (Gas6^−/− → WT and WT → Gas6^−/−) had an intermediate weight compared with Gas6^−/− → Gas6^−/− and WT → WT (n = 8). *P < .05. #P < .001. (C) Thrombi were also digested in proteinase K. Protein concentration evaluation confirmed the intermediate phenotype of chimeric mice (n = 8). #P < .001.