Integrated proteomic and transcriptomic investigation for the identification of new leukemia-associated markers. The leukemia surfaceome data set was reduced to 210 proteins identified in more than half of investigated leukemia cases (at least 11 of 19 cases; 58%) and probed against published gene expression signatures of B cells at different maturation stages in normal hematopoiesis (DMAP). One hundred eighteen genes represented in DMAP corresponding to the proteins of interest were ranked based on average expression in precursor B-cell populations (step 1). Eighty-three genes with average expression below 0 (green bar; #) were further ranked based on average gene expression in the cluster of B cells from later ontogeny stages (step 2) resulting in 33 gene IDs with expression below 0 (green bar; ##). Cell populations and lineages: 1, hematopoietic stem cells; 2, common myeloid progenitors; 3, megakaryocyte/erythroid progenitors; 4, erythroid cells; 5, megakaryocytes; 6, granulocyte/monocyte progenitors; 7, granulocytes; 8, monocytes; 9, eosinophils; 10, basophils; 11, dendritic cells; 12, precursor B cells; 13, naive and mature B cells; 14, natural killer cells; 15, T cells.