Analysis of integrin αIIbβ3 inside-out activation. Flow cytometric analysis of PE-JON/A binding (A) or FITC-labeled fibrinogen binding (B) to WT (black bars) and Pyk2-KO (white bars) platelets stimulated with the indicated doses of thrombin, AYPGKF, or convulxin (CVX). Data are expressed as means ± SD of 3-8 different experiments. ***P < .001; **P < .01