Differences in NK-receptor expression are intrinsic to mutant NK cells. (A) Schematic diagram depicting the experiment. 1:1 mixtures of fetal liver or BM cells, both gfp+, from WT mice and NKG2D-KO, NKp46-KO, or DKO mice were injected (intravenously) into lethally irradiated WT recipients (gfp-negative). FACS analysis was performed 8 to 12 weeks post transplant by gating on gfp+ NK cells. Further gating using NKG2D or NKp46 markers was used to distinguish donor NK cells of WT or mutant origin. (B-C) Percentages of NK cells expressing Ly49G2, Ly49A, DNAM-1, and c-Kit in the spleens of recipient mice reconstituted with WT/NKG2D-KO, WT/NKp46-KO, and WT/DKO precursor cell mixtures. (D) Percentages of CD11b/CD27 subsets of NK cells in recipient mice reconstituted with WT/NKG2D-KO (upper), WT/NKp46-KO (middle), and WT/DKO (lower) precursor cell mixtures. (E) Percentages of IFN-γ responding NK cells from chimeric mice stimulated with anti-NK1.1 in vitro. All data are representative of 3 independent experiments (n = 5-7 mice/genotype)