NKG2D and NKp46 are not necessary for NK-cell development. (A-B) Percentages of NK cells in the spleen and BM of WT and mutant mice are depicted for individual mice analyzed in 3 to 6 independent experiments; mean ±SD is shown for each genotype. Absolute numbers of NK cells in the spleen are shown in (B). (C-D) Representative fluorescence-activated cell sorting (FACS) profiles from a mouse of each genotype showing expression of the indicated markers (C), and percentages of cells expressing various marker combinations (n = 5 mice/genotype) (D). Data are representative of at least 4 independent experiments. (E) BM NK cells were stained for the cell-surface expression of DX5 and intracellular expression of the transcription factors T-bet and Eomes; median fluorescence intensities are indicated. Data are representative of 3 independent experiments.