Role of Coro1A in integrin biology. CSRM (A) and STED nanoscopy (B) of murine PMN upon Mn²⁺-induced adhesion to fibrinogen. (A) Coro1A (red), CD18 (green), and merge (yellow) in 2 different z-stack positions and in 1 orthogonal layer. Scale bar, 1 µm. Colocalization was calculated using the Pearson’s correlation coefficient (P_r). (B) Coro1A and CD18 in pseudocolors (colocalization in yellow) at surface proximal layer demonstrated by reflection pattern shown in transmission picture and intensity profile along segmented line. Partial (open circle) and complete (arrows) colocalization. Color scale, heat map. Scale bar, 5 μm. (C) Pull-down assay using synthesized peptides of the cytoplasmic tail of CD18 or scrambled control peptides incubated with different protein amounts of lysates of primary human PMNs (n = 3). (D) Subcellular localization of Coro1A (arrows) during trauma-induced PMN trafficking, that is, adhesion, intraluminal crawling, and transmigration in cremaster muscle whole mounts. Coro1A (green), CD31 (red), Ly6-G (blue). Scale bar, 10 µm. Coloc, colocalization; Scr, scrambled control peptides.