Effects of Hmga2 overexpression on hematopoietic precursors and progenitors in mice expressing Jak2^V617F. (A) Representative dot plots of flow cytometric analysis of erythroid precursors in the BM and spleens (SPL) of WT-vector, WT-Hmga2, Jak2^VF/+-vector, and Jak2^VF/+-Hmga2 mice (at 32 weeks after BMT) using surface markers CD71 and Ter119. Total numbers of erythroid precursor cells are shown in bar graphs as mean ± standard error of the mean (SEM) (n = 5-10). (B) Representative dot plots of flow cytometric analysis of megakaryocytic precursors in the BM and spleens of WT-vector, WT-Hmga2, Jak2^VF/+-vector, and Jak2^VF/+-Hmga2 mice using CD41 staining. Total numbers of CD41⁺ megakaryocytic precursors in the BM and spleens are shown in bar graphs as mean ± SEM (n = 5-10). (C) Representative contour plots of flow cytometric analysis of Lin^–Sca-1⁺c-kit⁺ (LSK), long-term HSC (LT-HSC; Lin^–Sca-1⁺c-kit⁺CD34^–CD135^–), short-term HSC (ST-HSC; Lin^–Sca-1⁺c-kit⁺CD34⁺CD135^–), multipotential progenitor (MPP; Lin^–Sca-1⁺c-kit⁺CD34⁺CD135⁺), common myeloid progenitor (CMP; Lin^–Sca-1^–c-kit⁺CD34⁺FcγRII/II^low), granulocyte/macrophage progenitor (GMP; Lin^–Sca-1^–c-kit⁺CD34⁺FcγRII/II^high), and megakaryocyte/erythroid progenitor (MEP; Lin^–Sca-1^–c-kit⁺CD34^–FcγRII/III^–) cells in the BM and spleens from WT-vector, WT-Hmga2, Jak2^VF/+-vector, and Jak2^VF/+-Hmga2 mice at 32 weeks after BMT. Total numbers of LSK, LT-HSC, ST-HSC, MPP, CMP, GMP, and MEP cells in the BM and spleens are shown in bar graphs as mean ± SEM (n = 8-10). *P < .05; **P < .005.