Enhancement of miR-193a levels by siAML1/ETO, 5-Aza treatment and ectopic miR-193a restores myeloid differentiation in SKNO-1 cells. (A) Relative qRT-PCR quantification of miR-193a level (*P = .004, #P = .006) and (B) flow cytometric analysis of CD11b expression in SKNO-1 cells at 144 hours after transfection with 100nM synthetic miR-193a, NC inhibitor or miR-193a inhibitor. NC, Negative Control. Histograms show 3 independent evaluations ± SD (*P = .005, #P = .008). (C) Relative qRT-PCR quantification of miR-193a level and (D) flow cytometric analysis of CD11b expression in SKNO-1 and SKNO-1-siA/E cells at 144 hours in the presence or absence of 2.5μM 5-aza after transfection with 100nM synthetic NC inhibitor or miR-193a inhibitor. Histograms show 3 independent evaluations ± SD. (E) Representative flow cytometric analysis of cell-cycle distribution of SKNO-1 cells transfected with vector or lenti-193a at 72 hours. (F) Morphologic analysis of SKNO-1 cells at 4 days after transfection with vector or Lenti-193a. Scale bar = 10 μm. (G) Analysis of CFU and (H) morphology in SKNO-1 cells during lenti-193a infection using methylcellulose culture system. WT indicates wild-type. Scale bar = 1 mm. Data are the representative of 3 independent experiments. (I) Flow cytometry analysis of apoptosis in SKNO-1 cells at 72 hours after transfection with 100nM synthetic miR-193a or NC. Histograms show 3 independent evaluations ± SD.