iNKT cells expanded from cord blood or thymus and freshly isolated iNKT cells are more prone to apoptosis than Vβ2 conventional T cells. (A) Apoptosis of expanded iNKT and Vβ2 T cells from blood and cord blood stimulated with anti-CD3, anti-FAS, or staurosporine. Data correspond to 2 experiments done in duplicate (error bars correspond to SD) with cells from 2 different donors (Nos. 7 and 14) and 2 different cord blood donors. (B) Apoptosis of expanded iNKT and Vβ2 T cells from 1 blood donor and thymocytes of 1 donor stimulated with anti-CD3 or anti-FAS. Data correspond to 1 experiment with assays done in duplicate (error bars correspond to SD). (C) Apoptosis of freshly isolated PBMCs from 4 different healthy donors (Nos. 4, 6, 9, and 12) stimulated with anti-CD3 for 8 hours. (D) Apoptosis of freshly isolated iNKT cells and Vβ2 T cells of PBMCs from 2 healthy donors (Nos. 9 and 13) left unstimulated for 72 hours. See also supplemental Figures 1 and 2.