Colocalization of VWF with CLEC4M. To verify that VWF binding colocalized with CLEC4M expression on the cell surface, CLEC4M-expressing cells were incubated with Humate-P (2 U/mL VWF) for 30 minutes, fixed, and labeled for CLEC4M (red), VWF (green), and DAPI (blue). Colocalization of VWF with CLEC4M is observed in yellow (merge). Images are representative of 3 to 4 separate experiments.