Pear1 controls Ptena, a regulator of thrombocyte production. (A) Western blot of Akt-P (Ser473) at 3 days postfertilization (dpf) in zebrafish after injection of control or PEAR1 MO. (B) Western blot for GFP and Akt-P in whole fish after treatment with LY294002 or DMSO, added at 30 hpf and analyzed at 3 dpf, with Akt as a loading control. (C) mRNA expression of Ptena and Ptenb by qRT-PCR 3 dpf after injection of control or PEAR1 MO, expressed as percentage of control (n = 3). (D) Western blot analysis of GFP, Akt-P, and Akt after injection of PTENa or PTENb MO 3 dpf after injection.