hOCT1 mRNA and protein expression. (A) hOCT1 expression for the hOCT1 variants in relation to the mock-transfected cells (KCLpcDNA). (B-C) Protein expression carried out by flow cytometry (B) and Western blotting with densitometry (C, which also shows blots with their loading control, Na⁺/K⁺ ATPase). In both panels B and C, data are expressed relative to the undeleted M420+M408 line, which was arbitrarily defined as 100%.