The full activation of the self-renewal program by MLL-Af4 depends on lymphoid lineage. (A) Structure of MLL-Af4 and MLL-CHD constructs. (B) Growth curve of human HSPCs expressing MLL-Af4/MLL-CHD in myeloid culture. One representative experiment of 3 is shown. (C) Flow cytometry analysis of CD33/CD19 expression of MLL-Af4/MLL-CHD cultures at week 5. (D) Cell surface maker analysis by flow cytometry of BM from primary NSG mice receiving MLL-Af4/MLL-CHD myeloid cells. (E) Representative flow cytometry analysis of secondary MLL-CHD ALL (see also supplemental Table 2). (F) Gene set enrichment analysis to assess the activation of HSC signature comparing MLL-Af4 cells with MLL-AF9 cells in myeloid or lymphoid lineage. NES, normalized enrichment score; TAD, transactivation domain.