p65 is an important regulator of gene expression in HSPCs. (A) RNA from sorted LSKF cells of individual wild-type or p65^hem−/− mice was used for microarray analysis. A heat map indicating changes of ≥1.5-fold with P ≤ .01 includes 689 genes. (B) Changes in gene expression were confirmed by quantitative reverse transcriptase-polymerase chain reaction. Data are representative of 3 separate experiments. (C) A representative table of relevant changes in gene expression. (D) The gene expression profile of p65^−/− HSCs was compared with those of (top) long-term HSCs, (middle) late hematopoietic progenitors, and (bottom) dividing hematopoietic cells by Gene Set Enrichment Analysis using curated gene lists.