Figure 5
Figure 5. IL-33–induced peritoneal neutrophil influx is mast cell–dependent. (A) Flow cytometry plots of c-Kit+ mast cells and Ly-6G+ neutrophils in peritoneal fluid from wild-type mice, Wsh/Wsh-mice, Wsh/Wsh mice reconstituted with wild-type BMMCs and Wsh/Wsh mice reconstituted with T1/ST2−/− BMMCs and injected intraperitoneally with 0.1 μg IL-33. Representative plots are shown. (B) Quantification of panel A (n = 4-5). (C) Percentage of mast cells in the peritoneum of wild-type, Wsh/Wsh-mice, and Wsh/Wsh mice reconstituted with wild-type or T1/ST2−/− BMMCs (n = 5-11). Values are presented as mean ± SEM, *P < .05, ns = not significant.

IL-33–induced peritoneal neutrophil influx is mast cell–dependent. (A) Flow cytometry plots of c-Kit+ mast cells and Ly-6G+ neutrophils in peritoneal fluid from wild-type mice, Wsh/Wsh-mice, Wsh/Wsh mice reconstituted with wild-type BMMCs and Wsh/Wsh mice reconstituted with T1/ST2−/− BMMCs and injected intraperitoneally with 0.1 μg IL-33. Representative plots are shown. (B) Quantification of panel A (n = 4-5). (C) Percentage of mast cells in the peritoneum of wild-type, Wsh/Wsh-mice, and Wsh/Wsh mice reconstituted with wild-type or T1/ST2−/− BMMCs (n = 5-11). Values are presented as mean ± SEM, *P < .05, ns = not significant.

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