Figure 3
COX inhibitors reduce the percentages of lin-Sca-1+c-Kit+ population in AE-expressing BM cells without inducing apoptosis. The lin− BM cells from AE/Mx1-cre mice were cultured in methylcellulose-based medium in the presence of vehicle (DMSO), INDM (50 μM), or NS-398 (50 μM), and were replated as previously described. These cells were analyzed for cell cycle phases (A), apoptosis (B), and percentages of lin−Sca-1+C-Kit+ cells (C) by flow cytometry after 1 to 3 weeks of treatment. Two independent experiments showed similar results. The results of a duplicate experiment are shown in supplemental Figure 2.

COX inhibitors reduce the percentages of lin-Sca-1+c-Kit+ population in AE-expressing BM cells without inducing apoptosis. The lin BM cells from AE/Mx1-cre mice were cultured in methylcellulose-based medium in the presence of vehicle (DMSO), INDM (50 μM), or NS-398 (50 μM), and were replated as previously described. These cells were analyzed for cell cycle phases (A), apoptosis (B), and percentages of linSca-1+C-Kit+ cells (C) by flow cytometry after 1 to 3 weeks of treatment. Two independent experiments showed similar results. The results of a duplicate experiment are shown in supplemental Figure 2.

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