Figure 1
AE induces COX gene expression in mouse BM cells. (A-B) WT mouse BM cells were transduced with GFP or AE-GFP retrovirus. GFP-positive cells were sorted and plated in methylcellulose medium to form colonies. The cells of the colonies were then pooled for RNA isolation and for replating each week. The expression levels of Cox-1 (A) and Cox-2 (B) were measured by real-time RT-PCR. Relative expression is calculated as fold change compared with GFP-retrovirus transduced BM cells harvested in week 1. (C-D) BM cells isolated from WT and AE/Mx1-Cre mice were cultured, and the expression levels of Cox-1 (C) and Cox-2 (D) were measured and calculated as previously described. (E-F) BM cells isolated from WT and AE/Mx1-Cre mice were depleted of lineage-committed cells. The lin− cells were cultured and the expression levels of Cox-1 (E) and Cox-2 (F) were measured and calculated as previously described. Data represent as mean ± SD; Student t test. *P < .05; **P < .01.

AE induces COX gene expression in mouse BM cells. (A-B) WT mouse BM cells were transduced with GFP or AE-GFP retrovirus. GFP-positive cells were sorted and plated in methylcellulose medium to form colonies. The cells of the colonies were then pooled for RNA isolation and for replating each week. The expression levels of Cox-1 (A) and Cox-2 (B) were measured by real-time RT-PCR. Relative expression is calculated as fold change compared with GFP-retrovirus transduced BM cells harvested in week 1. (C-D) BM cells isolated from WT and AE/Mx1-Cre mice were cultured, and the expression levels of Cox-1 (C) and Cox-2 (D) were measured and calculated as previously described. (E-F) BM cells isolated from WT and AE/Mx1-Cre mice were depleted of lineage-committed cells. The lin cells were cultured and the expression levels of Cox-1 (E) and Cox-2 (F) were measured and calculated as previously described. Data represent as mean ± SD; Student t test. *P < .05; **P < .01.

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