Cytotoxic activity of hTERT-siTCR–transduced CD8⁺ T cells against ATL/HTLV-I–infected cell lines. (A) Cytotoxic activity of hTERT-siTCR–transduced CD8⁺ T cells against HLA-A*24:02⁺ or HLA-A*24:02⁻ ATL/HTLV-I–infected cell lines was tested in ⁵¹Cr-release assays at the indicated E/T ratios. All tested ATL/HTLV-I–infected cell lines overexpressed hTERT mRNA and protein, as shown in Figure 1. (B) Effect of HLA class I and class II blockade on the cytotoxic activity of hTERT-siTCR–transduced CD8⁺ T cells against ATN-1 and TL-Su was tested in ⁵¹Cr-release assays at an E/T ratio of 5:1.