Figure 1
![Figure 1. The region between CALM aa 520-583 is essential for CALM-AF10–mediated immortalization. Murine bone marrow progenitor cells were transduced with retroviral constructs expressing CALM-AF10 proteins schematically shown on the left. Bar graph (right) indicates the number of colonies generated per 10 000 cells seeded in second and third passage methylcellulose cultures. The mean ± SEM are shown from duplicate samples analyzed in 3 [CALM(1-552)-AF10], 4 [CALM(520-583)-AF10], or 5 [Vector, CALM-AF10, and CALM(1-583)-AF10] independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/121/23/10.1182_blood-2012-06-435792/4/4758f1.jpeg?Expires=1723728040&Signature=W5HFdUB6hLtUJHwkKxkL5Q1QNyVBbkG8omXkguIuHzf~sK-DR7ZBqWSsXIdm8ovgfB9M6mdx2Syx8fj5ITE9CW37LYToANc~FHR-DcoLOUYifyrPdTTcWn~7PXpocFh9cGHhxMPLMh5NJOctgvdD3fWm~YTa5nQ54H6D06Yjb-SidYlgbBgeah1tUD82jU8I3nNz4Vu2IrddPTRTCOaDn-Yzdtbp6R4pCLGld8z0JX~RwoQ5Q-bobmt5bpeF24BjgSuSY7COD0QciUfaevGTlNmm-A~YzWYWY9A-29RW5JWkadIk8~72qk7GWwI9a7gu~6dCez4HSCWEOLT8qfQl1g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The region between CALM aa 520-583 is essential for CALM-AF10–mediated immortalization. Murine bone marrow progenitor cells were transduced with retroviral constructs expressing CALM-AF10 proteins schematically shown on the left. Bar graph (right) indicates the number of colonies generated per 10 000 cells seeded in second and third passage methylcellulose cultures. The mean ± SEM are shown from duplicate samples analyzed in 3 [CALM(1-552)-AF10], 4 [CALM(520-583)-AF10], or 5 [Vector, CALM-AF10, and CALM(1-583)-AF10] independent experiments.
